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Validation of ITS3 Primer for the Plant Vigna Radiata

Sowndarya Murali*, Prem Kumar A

 Legumes are the third largest flowering family and are the second only to cereals in their importance for human nutrition. Mung bean and common bean are legumes which are rich in nutrients such as proteins, oils and carbohydrate. DNA barcoding, a system for fast and accurate species identification will make ecological system more accessible. Plant DNA barcoding have a huge role in conserving endangered species and in assessment of biodiversity hotspots and also to monitor the international trade of the rare species apart from the routine identification. In this preliminary study, the ITS primers were evaluated and found to be the best for the selected plant Vigna radiata (L.). ITS primers 3 was found out to be a suitable barcode.