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Validated Spectophotometric Methods for the Estimation of Nebivolol

S. M. Malipatil, M. Deepthi, S. K. Patil and Prakash S. Sarsambi

Two simple, sensitive and rapid spectrophotometric methods (Method A and Method B) in the visible region have been developed for quantitative estimation of nebivolol from bulk drug and its pharmaceutical formulations (Tablets). Method A is based on oxidation followed by coupling of 3-methyl- 2-benzothiazolinone hydrazone (MBTH) with nebivolol in presence of ferric chloride to form bluish green color chromogen, which exhibits absorption maximum at 655 nm. The Beer’s law was obeyed in the concentration range of 1 - 5 μg/mL. Method B is based on oxidation followed by complex formation with potassium ferricyanide with nebivolol in presence of ferric chloride to form a stable blue colored chromogen with maximum absorption at 728 nm. The Beer’s law was obeyed in the concentration range of 5 - 25 μg/mL. The results of the analysis for both the developed methods have been validated statistically by recovery studies. The results obtained with the proposed methods (Method A and Method B) were compared with UV spectrophotometric method in methanol at 282 nm.