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Urinary protein thiols in different grades of proteinuria

Mungli Prakash, Jeevan K.Shetty, Sharanbasappa M.Awanti, Sambit Dash, Bijay K.Barik, Abhirup Sarkar, Ravindra Prabhu

Total thiol status of plasma, especially thiol (-SH) groups over protein contributes maximumto the plasma antioxidant status of the body. Serum protein thiols were found to be decreased in various disease conditions including chronic renal failure patients. Only few studies determined the levels of urinary protein thiols in disease conditions. The current study was designed to know the levels of urinary protein thiols in patients with different grades of proteinuria. The study was conducted on urine of 40 healthy controls and 61 cases with proteinuria. Based on proteinuria cases were further divided into two groups; group I -microproteinuria (150-300 mg protein/d), 32 cases, group II-frank proteinuria (>300mg protein/d), 29 cases. Urinary thiol levels were determined by spectrophotometric method using dithionitrobenzoic acid (DTNB). We found significant decrease in urinary thiols in group I cases (p<0.01) and in group II cases (p<0.01) compared to healthy controls. In conclusion, urinary thiols are decreased according to the grades of proteinuria.