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The extraction for the different polarity fractions of the endophytic fungi of Arthrinium sp L1 from Pleioblastus amarus and biological activity

Yu-xian Li, Yin Lin, Wei Han, Ke Yuan

This essay researches into the extraction of the different polarity parts of the endophytic fungi of Arthrinium sp L1 from Pleioblastus amarus and its biological activities like bacteriostatic activity, antioxidant activity and cytotoxic activity. separate and purify the endophytic fungi from the plant bitter bamboo, and determine it as Arthrinium sp L1; after fermenting it, extract the following different parts respectively, the petroleum ether, ethyl acetate and n-butyl alcohol. Establish the slanting-tube method to evaluate how the different parts of Arthrinium sp L1 affect the in-vitro bacteriostasis of the phytopathogens through determining the value of MIC; determine the in-vitro antioxidant activity of the different parts of Arthrinium sp L1by using the following three methods DPPH, ABTSï¼Ã‚‹, and FRAP, have a preliminary screening for the cytotoxic activity of the different parts of Arthrinium sp L1 by using larva as the objects and the fatal-to-prawn bioactivity experiment. The different parts of Arthrinium sp L1 all have certain bacteriostatic activity, antioxidant activity and cytotoxic activity, and they all have obvious dose-effect relationship. Among them, the n-butyl alcohol extract has the strongest bacteriostatic activity for Fusarium graminearum and Sclerotinia sclerotiorum, and the ethyl acetate extract has the strongest antioxidant and cytotoxic activities.