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Synthesis of Silver Nanoparticles from Leaf Extract of Olive and Fig with Silver Nitrate and Effect on ECTO-5- Nucleotidase (5-NT), ADA and AMPDA Enzymes in Sera of Arthrosclerosis Patients

Ahlam M. Farhan, Atheer Awad Mehde, Wesen Adel Mehdi, Rasha A. Jassim, Nafeesa J. Kadhim and Nagham A. Jasim

There is an increasing commercial demand for nanoparticles due to their wide applicability in various areas such as electronics, catalysis, chemistry, energy, and medicine. Metallic nanoparticles are traditionally synthesized by wet chemical techniques, where the chemicals used are quite often toxic and flammable. In this work, we describe a cost effective and environment friendly technique for green synthesis of silver nanoparticles from 1 mM AgNO3 solution through the extract of leaf extract of olive and fig as reducing as well as capping agent. Nanoparticles were characterized using UV-Vis absorption spectroscopy, atomic force microscopy (AFM) and scanning electron microscope (SEM). Also the effect of these nanoparticles on adenosine aminohydrolase (ADA), AMP-aminohydrolase (AMPDA) and Ecto- 5'-nucleotidase (5'-NT) activities in sera of 80 patients with atherosclerosis and 70 control person. The AFM and SEM analysis showed the average particle size of 102.76 nm with olive, 88.23 nm with fig, while the sizes of silver nanoparticles from mix fig and olive as a ratio 1:1, 1:4, 1:6 and 3:5 were (103.00, 77.49, 103.58 and 97.48) nm, respectively. The current study suggest that the nanoparticles are a good inhibitory effects on 5'-NT, ADA and AMPDA enzymes in sera of control and patients with arthrosclerosis.