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Spectrophotometric determination of eflornithine hydrochloride as active pharmaceutical ingredient using sodium1,2-aphthoquinone-4-sulfonate as the derivative chromogenic reagent

Amit Kumar, S.P.Venkatesh Prasad, S.Mohan, Pradeep Kumar


A simple highly sensitive spectrophotometric method was developed for the quantification of eflornithine hydrochloride (2-difluoromethyl-DL-ornithine; DFMO). The method involves the reaction of the target compound with sodium1,2-naphthoquinone-4-sulfonate (NQS) reagent at specific pH 5.6 to produce a wine red color chromogen. The derivative chromogen exhibits absorption maxima at 498nm. At the specific pH of the reaction where no degradation may occur with that medium the proposed method can be utilized as a stability indicating assay. The different experimental parameters affecting the derivatization reaction were carefully studied and incorporated into the procedure. Under the described conditions the proposed method is linear over the concentration range of 10-70mcg/ml and the coefficient of determination were >0.999(n=6) with a relative standard deviation of 1.98% (n=6). The average recovery of the target compound is 99.28%with a limit of quantification (LOQ) of 0.52mcg/ml and the limit of detection (LOD) 0.172mcg/ml. Themechanismof the derivatization reaction is proposed and advantages of the proposed method are discussed.


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  • CASS
  • கூகுள் ஸ்காலர்
  • ஜே கேட் திறக்கவும்
  • சீனாவின் தேசிய அறிவு உள்கட்டமைப்பு (CNKI)
  • CiteFactor
  • காஸ்மோஸ் IF
  • எலக்ட்ரானிக் ஜர்னல்ஸ் லைப்ரரி
  • டைரக்டரி ஆஃப் ரிசர்ச் ஜர்னல் இன்டெக்சிங் (DRJI)
  • ரகசிய தேடுபொறி ஆய்வகங்கள்
  • ICMJE

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