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Spectrophotometric Determination of Amisulpride

S. Humairaa, A. K. Deya, S. Sanaullahb and S. A. Raju

Three simple and sensitive spectrophotometric methods (A, B and C) have been developed for the quantitative estimation of amisulpride in bulk drug and pharmaceutical dosage forms. Method A is based on the diazotisation of amisulpride with nitrous acid followed by its coupling in situ with N-(1- naphthyl) ethylenediamine dihydrochloride to form pink coloured chromogen exhibiting absorption maximum at 523.5 nm and Beer’s law is obeyed in the concentration range of 2-10 μg/mL. Method B is also based on the diazotisation of drug with nitrous acid followed by its coupling insitu with -naphthol to form orange coloured chromogen exhibiting absorption maximum at 482.5 nm and Beer’s law is obeyed in the concentration range of 2-10 μg/mL. Method C is based on the reaction of amisulpride with Folin-Ciocalteau (FC) reagent in alkaline condition to form stable blue coloured chromogen with absorption maximum at 736 nm and Beer’s law is obeyed in the concentration range of 10-50 μg/mL. The results are compared with those obtained using UV spectrophotometric method in 0.1N HCl at 226.5 nm