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Simultaneous RP HPLC determination of bambuterol HCl in pharmaceutical preparations

V.V.Vaidya, A.Y.Desai, P.S.Barde, Shikha M.N.Roy, K.V.Mangaokar

A simple, fast and precise reversed phase high performance liquid chromatographic method is developed for the simultaneous determination of bambuterol HCl using mebeverine HCl as an internal standard. Chromatographic separation of these two drugs was performed on a inertsil C18 column (250mm4.6 mm, 5m) as stationary phase with a mobile phase comprising of 0.02Mpotassiumdihydrogen orthophosphate: acetonitrile (50:50 v/v), at a flowrate of 0.7mlmin-1 andUV detection at 215nm. The Retention time of Bambuterol HCl and Mebeverine HCl were 3.17 min and 4.88 min respectively. The proposed method was validated for linearity, accuracy, precision, LOD, LOQ. Linearity, accuracy and precision were found to be acceptable over the ranges of 100-300 -gml-1 for bambuterolHCl. It can be conveniently adopted for routine quality control analysis.