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Screening Of Human Eye Shape Gene Polymorphism By Polymerase Chain Reaction

Y.Farida Zuraina, H.Khairedza Rahmi, O.Khairul, S.Rita Rohaiza

Rape victims have usually poor recollection of their rapist. In certain cases where victim is lucid, facial reconstructions through sketching are done by the help of trained forensic sketchers. Despite assistance, minor facial characteristics which would improve overall facial structure have always been overlooked due to the poor lighting at the site of incidence or the poor recollection of the victim. Since rapist DNA can usually be obtained from the rape site, a study was conducted to look at relationship between the eye shape and polymorphism of the eye lid gene. A total of 51Malays, 23 Chinese and 18 IndiansDNAwere extracted using QIAampDNAbloodmini kit (QIAGEN,HildenGermany).Amplification of the eye gene lociwas done usingGDB11509038For andGDB11509038 Rev primers. Later polymorphism was determined using restriction enzyme, BsmAI. Results indicated that 86 samples shown positive amplification with GDB11509038 primer pair. The polymorphism patterns are found to be inconsistent with eye shapes.