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Screening and production of cellulase by fungal culture isolated from soil contaminated with cattle dung

G.Nandana, A.Sridevi, G.Narasimha

In this study a cellulolytic fungi was isolated from soil contaminated with cattle dung for cellulase production. The cellulolytic property of fungal culture was confirmed by plate screening assay based on formation of yellow zone surrounding of fungal culture on Congo red Agar medium plate. The cellulase enzyme was produced by fungal culture in submerged fermentation. The protein content in the fungal filtrate was estimated with 1660 g/ml. The cellulase activities, Filterpaperase (FPase, 1.79U), Carboxymethyl cellulase (0.68U) and â-glucosidase (0.2 U) was exhibited by fungal culture isolated from with cattle dung contaminated soil.