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Quantification of puerarin from Pueraria tuberosa DC by using high performance liquid chromatography

Harshad P.Mhaske, Vikas V.Vaidya, Shveta H.Mhaske, Pramod N.Pabrekar, Maharudra B.Kekare

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method has been developed for the determination of puerarin in root powder of Pueraria tuberosa DC. The analysis was done with ThermoHypersilBDS C18 column (150mm4.6mmi.d., 5mparticle size) as stationary phase at a wavelength of 254 nmfor detection and determination. The proposedHPLCmethod was validated for linearity, accuracy, precision and limit of quantitation. The validated HPLCmethod can be used for a routine quality control analysis of Pueraria tuberosa DC. and quantitative determination of puerarin from root powder of Pueraria tuberosa DC.