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Partial Purification of CAT and Study Anti-Oxidant Activity of Grape Seeds Vitis vinifera

Farhan LO, Shwayel FW and Talal GK

Grape (Vitis vinifera L.) is the chief fruit crop in the world. It is one of the fruits which are grown from ancient time; Grapes contain about 80% water, so it is delicious, low‐calorie snack or dessert. During making juice from a grape, high quantities of the grape pulp, seeds, and skin will be as food for animals. Hence, the study was aimed to Measure of MDA, TAC, CAT and Vit C in different types of grape, and Partial purification of CAT from Red Globe. TAC, MDA, Vitamin C, Total protein and cat had been measured in seven kinds (Christmas rose, Italia, emperor, Muscat of Alexandria, early Muscat, red Globe, and black rose) of grape seeds and cat enzyme was purified from Red Globe grape seeds. The results showed that the Christmas rose, red globe and black rose have the highest vitamin C content and Muscat of Alexandria has the lowest vitamin C content from this analysis. While Muscat of Alexandria has the highest MDA content and Emperor has the lowest MDA content from this analysis, and Early Muscat has the highest TAC content and Black Rose has the lowest TAC content from this analysis. In addition purification of Cat from Red Globe grape seeds by gel filtration Sephadex G-100 and EDTE-Sepharose.