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Optimizing the extraction of total saponins from Ornithogalum Caudatum Ait. by response surface methodology

Xiang Zou, Zhong-YuanQu, Lin Lin, Yu-Meng Wang, Yu-Bin Ji

To optimize the extraction process of total saponins from Ornithogalum Caudatum Ait. (OC), ethanol was selected as the extraction solvent and the extraction rate of total saponins was selected as evaluation index. Response surface methodology were used to optimize the extraction process ofOC saponins. The result showed that the best extraction process of total saponins fromOC was as follows: the temperature was 65 °C, the ethanol concentration was 82% and the extraction time was 4.3 h. Under the optimum condition, the actual extraction rate of total saponins from OC was 0.6743%which was higher than that under the other experimental conditions. From the above, we could draw the conclusion that response surface methodology was a feasible and effective method for optimizing extraction process of the OC total saponins and the results could provide reference for the industrial production of total saponins from OC.