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Metabolic and biochemical evaluation of Weissella hellenica SKkimchi3 that produces different amount of exopolysaccharide (EPS) according to kind of sugars

Bo Young Jeon, Doo Hyun Park

Growing cells of W. hellenica SKkimchi3 produced significantly higher exopolysaccharide (EPS) fromsucrose than glucose, fructose, and glucosefructose mixture. Crude enzymes isolated from SKkimchi3 grown on sucrose, glucose, and fructose commonly catalyzed higher EPS synthesis from sucrose than glucose and fructose. The affinity and activity (Km and Vmax) of crude enzymes for sucrose were relatively higher than that for glucose and fructose. The pattern of 2D-SDS-PAGE for total soluble protein isolated from SKkimchi3 grown on sucrose was similar to that grown on glucose, but the expression of some proteins differed. Sucrose may not be a factor to induce metabolic activity of SKkimchi3 for EPS production but may be an optimumsubstrate to induce enzymatic activity for EPS synthesis.