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Isolation, characterization and preservation of toluene degraders from soil subjected to biofiltration studies

Swaminathan Detchanamurthy, Peter Alan Gostomski, Abishek Muralidhar

Amicrobial community of a soil differential biofilter reactor treating toluene vapors was investigated in this work. Strains were isolated and checked for toluene-degradation activity. Following basic primary identification, the secondary identification of the isolated strains was carried out using 16S rRNA and 18S rRNA gene-sequence comparison which showed that they belonged to 4 genera: Pseudomonas, Stenotrophomonas, Aspergillus, and Ochrobactrum. Thisworkwill help the current biofiltration researchers in selecting the appropriate toluene degraders in developing biofilmbased biofilter reactors for the effective treatment of toluene.  2014 Trade Science Inc. - INDIA