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In vitro organogenesis and plantlet regeneration of pigeonpea [Cajanus cajan (L.) Millsp.]

Mustafa Abul KalamAzad, Muhammad Nurul Amin

Adventitious shoot regenerationwas achieved fromepicotyl and internode explants of in vitro grown seedlings of Cajanus cajan.Maximumnumber of multiple shoot formation was obtained by culturing the above explants on MS medium containing 1.0 mg/l BA and 0.5 mg/l NAA. The microcuttingswere developed to complete plantlets after transferring them to the rooting media supplementation with auxins namely, IBA, NAA or IAA. Nevertheless, 0.1-0.2 mg/l IBA and NAA was found to be most suitable for root induction. The microplantswere successfully established in the soil under natural environment with about 75% survival.