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Immunocomplex formation and radioactive antigen displacement in C-peptide RIA

J.L.Moreno Frigols, C.Olivas Arroyo

The double antibody radioimmunoassay (RIA) is one of the main methods used in the analytical determination of C-Peptide. The influence of initial concentrations of labelled (M), the binding of the immunocomplex (PM) to the second antibody (J) immobilised on a bead, and the replacement of M by Q in the PMJ immunocomplex have been studied. In order to study the effect of such variables, 30 experimentswere conducted and an explanation based on a physical-chemical model is proposed. The model used successfully reproduces the results.