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Fluorescence Resonance Energy Transfer Quenching On Concentration Determination Of Sudan I In Food

Baosheng Liu, Zhaozhen Cao, Zhichao Liu, Hang Liu

Depending on the isochromatism between the fluorescence of acridine Yellow (AY) and the absorption of Sudan I, at λex/λem = 280/495 nm, Sudan I absorbed the fluorescence of AY effectively in the medium of ethanol, which made AY quench, so we built the new way to determine the concentration of Sudan I. The range of determining concentration of Sudan I was 5.0×10-7 ~ 3.5×10-5 mol l-1. The detection limit of the method was up to 1.82×10-7 mol l-1 The method had applied to the determination of Sudan I in capsicum powder and chili sauce. The relative standard deviation was 0.8 ~ 2.7 % for 6 parallel determination of Sudan I samples and the recovery of Sudan I was 89.5 ~ 109.0 %.