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Effect of the Cuttlefish (Sepia officinalis) Pure Ink and the Muscle Extract on Cholestrol Level of Wister Rats Consuming a High Fat Diet: Biotechnological Application

Benchegra-Rabhia K, Zouggar AM, Dida-Taleb N, Bey BHM and Abi-Ayad SMA

Fisheries co-products are relatively high in some natural biological active components. The presented study attempts to investigate on the effect of natural pure ink and the muscle extract of the cuttlefish (Sepia officinalis) on Cholestrol level of Wistar Rats consuming a high fat diet. The effects of the nutrient intake lipid rich on hypercholesterolemia was determined by a 30-day experiment, in 24 male rats of Wistar strain, aged six weeks and weighing about 200 g. The observed rats are divided into 4 homogeneous groups of six (no: 6); one group receives throughout the experiment a commercial standard diet; the other 03 groups are subjected to a ten-day adaptation phase with a standard commercial diet supplemented with lipids (30% animal fat) (high fat diet: R-HL). After a period of 30 days experiment, a group still consumed the same diet R-HL, the 2nd observed group consumed the HM R-HL supplemented with 250 mg/kg of muscle hydrolysate cuttlefish, and the 3rd EP group consumes the R-HL supplemented with 250 mg/kg pure ink of the cuttlefish. At the 30th date of experiment, the results have shown that the R-HL supplementation of HM and EP has no effect on the contents of total lipids in serum and liver in four groups of rats. However, it modifies their contents in different lipids (total cholesterol, triglycerides, phospholipids). They also cause a 20% increase in the levels of serum proteins in the HM group compared to the HL group. Furthermore, assessment of lipid peroxidation by measuring the TBARS in serum and liver reveal a reduction of 50% in the HM group compared to the HL group. It is observed that some of bioactive components in muscle hydrolysates and pure ink of the cuttlefish (PUFA and bioactive peptides) studied possess considerable metabolic activities (anti-inflammatory and antioxidant). In conclusion, the study claims that the effect of Muscle Hydrolisate Supplementation (MHS) and natural pure ink of the cuttlefish (Sepia officinalis) have a significant effects on lowering Cholestrol level, which will be interesting for an industrial development, for their role in prevention against cardiovascular complications associated with hypercholesterolemia.