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Effect of metal carbonates on succinate production by Actinobacillus succinogenes in batch fermentation

Apichai Sawisit, Supaluk Seesan, Sunthorn Kanchanatawee, Sirima Suvarnakuta Jantama, Kaemwich Jantama

In the present study, the effect of metal carbonates (CaCO3, MgCO3, MnCO3, Na2CO3 and K2CO3) on the growth and succinate production by Actinobacillus succinogenes 130ZT was examined in 100-mL anaerobic bottles using a glucose-based medium. Initial glucose concentrations and metal carbonate in the form of its chlorides, sulphates, and phosphate were studied. The strain initially produced 7.75 g/L of succinate with a yield of 68%from 15 g/L of glucose within 24 h. However, when process optimization was employed, 10.85 g/L of succinate was produced in the same medium supplemented with 60 mMMgCO3with a succinate yield of 85%. As a result, the titer and yield of succinate were increased by 40% and 31%, respectively. Based on this study, the supplementation of MgCO3 in the cultured mediumsuggests an effective strategy for improving the succinate production in A. succinogenes 130ZT.