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Downstream processing studies for pullulan recovery in solid state fermentation using asian palmyra palm kernel - inexpensive substrate

K.R.Sugumaran,V.Ponnusami

Production of industrially important biopolymer, pullulan was investigated using asian palmyra palmkernel in solid state fermentation.After fermentation, polysaccharide was recovered by harvesting the cells at 121p C in autoclave, centrifugation followed by solvent precipitation. Recovery of pullulanwas tried with single step solvent precipitation method. Screening of solvents such as acetone, isopropanol, ethanol and butanol on pullulan recovery was studied. After screening the suitable solvent, effects of volume ratio of solvent & supernatant and precipitation time on pullulan recovery were carried out.