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Development and validation of HPLC method for the simultaneous determination of Perindopril and Indapamide in pharmaceutical formulations

M.A.El Kawy, K.M.Younes, Shaimaa M.Ali


A simple isocratic HPLC method has been developed and subsequently validated for simultaneous determination of Perindopril and Indapamide in pharmaceutical dosage forms. The method employs an Inertsil ODS C18 column, 5 ì, 250 mm x 4.60mm id with flow rate of 1.5 ml/min using UV detection at 215nm. The separation was carried out using a mobile phase consisting of potassium di hydrogen phosphate buffer having pH 5 and acetonitrile in the ratio of 60:40 respectively. The retention time for Perindopril and Indapamide was found to be 2.85 min and 3.85 min respectively. A linear response was observed over the concentration range of 5.0-40.0 µg / ml and 2.5 - 15.0 µg / ml for the assay of Perindopril and Indapamide with correlation coefficient of 0.9992 and 0.9994, respectively. The results of analysis were validated stastically and by recovery studies. Hence the proposed method was found to be accurate, precise, reproducible and specific and can be used for simultaneous analysis of these drugs in tablet formulations.


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  • CASS
  • கூகுள் ஸ்காலர்
  • ஜே கேட் திறக்கவும்
  • சீனாவின் தேசிய அறிவு உள்கட்டமைப்பு (CNKI)
  • CiteFactor
  • காஸ்மோஸ் IF
  • எலக்ட்ரானிக் ஜர்னல்ஸ் லைப்ரரி
  • டைரக்டரி ஆஃப் ரிசர்ச் ஜர்னல் இன்டெக்சிங் (DRJI)
  • ரகசிய தேடுபொறி ஆய்வகங்கள்
  • ICMJE

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