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Determination of ketoprofen enantiomers in plasma and exudate by liquid chromatography-Mass spectrometry

V�?�?�?�?�?�?�?­ctor Hormaz�?�?�?�?�?�?�?¡bal, �?�?�?�?�?�?�?�?yvin �?�?�?�?�?�?�?�?stensvik

A liquid chromatographic-mass spectrometrymethod for the determination of ketoprofen enantiomers in swine/cat plasma and exudate is described. Plasma /exudate proteins were precipitated with acetone-trichloroacetic acid and centrifuged. The supernatant (~ 500uL) was filtered through a centrifuge filter tube before it was diluted and injected into the LC-MS/ MS. The recovery for both R and S ketoprofen varied from 97 to 98% in plasma and exudate. The limits of quantification of R and S were 10 ng/mL and the limit of detection was 5 ng/mL for both R and S in plasma and exudate, based on 0.5 mL swine plasma or exudate.  2013 Trade Science Inc. - INDIA.