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Derivative spectrophotometric method for determination of diacerhein and aceclofenac in pharmaceutical formulations

Kirti S.Topagi, Mohit G.Dewani, Ashwini R.Madgulkar, Mrinalini C.Damle

A simple, accurate and precise spectrophotometric method was developed for simultaneous estimation of Diacerhein and Aceclofenac in tablets by using first order derivative zero-crossing method. Aceclofenac showed a zero crossing point at 275.8 nm while Diacerhein showed a zero crossing point at 293.4 nm. The dA/dë was measured at 293.4 nm for Aceclofenac and 275.8 nm for Diacerhein and calibration curves were plotted as dA/dë versus concentration, respectively. The method was found to be linear in the range of 10-50 ìg/mL for both the drugs. The limit of detection was found to be 0.48 and 0.53 ìg/mL for Aceclofenac and Diacerhein respectively. The limit of quantitationwas 1.46 and 1.6 ìg/mL forAceclofenac and Diacerhein respectively. The method was successfully applied for simultaneous determination ofAceclofenac and Diacerhein in formulation.