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Comparison of ELISA and HPLC techniques in determination of AFB1 in feedstuff

Mohamad Sadegh, Parya Rahnama, Ali Mohamadi Sani

This study aimed to determine the aflatoxin B1 (AFB1) levels in feed-stuff samples. For this purpose, 90 animal feed samples including barley, wheat bran,wheat pulp, canolameal, safflowermeal, cottonseedmeal and sunflower meal were randomly obtained from retail stores in Mashhad-Iran and analyzed by both enzyme linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) techniques. Results showed presence of AFB1 in 33 (36.67%) and 10 (11.11%) samples analyzed by HPLC and ELISAtechniques, respectively. The average±SD concentration ofAFB1 in the sampleswas 2.21±2.25 and 10.76±0.86 µg/kg for HPLC and ELISAtechniques, respectively. The range of contamination reported 0.34- 5.81 and 9.5-12.6 µg/kg for the mentioned techniques respectively. ELISA showed reliability and a high correlation with HPLC of 0.93 indicating its potential for aflatoxin screening in animal feed samples. However, sensitivity and specificity of HPLC was higher than ELISA method.