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Comparative studies of bromelain extraction- conventional vs ionic liquid based aqueous two phase extraction

Senthilkumar Rathnasamy, R.Kumaresan

Bromelain is a major proteinase, isolated from pineapple (Ananas comosus). In the plant, bromelain is accumulated in the entire plant to different extent and properties depending on its source. The objective of present study was to compare the extraction of bromelain present in stem juice of the plant using Ionic liquid (IL) based aqueous two phase (ATP) and Reverse micellar(RM) system. Separation and purification of high value bio-molecules using IL based ATP extraction is an emerging green separation process. Here the Phosponium based ILATP system was used and it is the best alternative for the conventional organic solvent extraction and polymer based ATP systems. The current study is to compare the partition coefficient of bromelian fromthe stemjuice of Ananas comosus and the same was compared with reverse micellar system. The resulting protein was purified on gel filtration chromatography column and it is confirmed with SDS page analysis. From the results it was found that IL based ATP system is highly selective and yield higher than the reverse micellar system.