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Chromatographic techniques for quantification of sterol from Mimosa pudica Linn. whole plant powder

P.A.Champanerkar, V.V.Vaidya, M.B.Kekare

A simple, precise, accurate and reproducible chromatographic methods has been established for quantification of -sitosterol in whole plant powder of Mimosa pudica Linn. The amount of -sitosterol in whole plant powder of Mimosa pudica Linn. was found to be 0.07mg. A methanol extract of the whole plant powder was used for the experimental work. Separation was performed using two different chromatographic techniques namelyHPTLC and HPLC. -sitosterol response was found to be linear over the range 1g/ mL to 20g/mL in case of HPTLC and 0.5g/mL to 20g/mL in case of HPLC. Both the methods were validated and can be used for a routine quality–control analysis of Mimosa pudica Linn. whole plant powder and quantification of -sitosterol.