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Characterization of 31-kda, Tubulin -1 chain like protein of Euphorbia nivulia Buch.-Ham. Latex

Shamkant B.Badgujar, Raghunath T.Mahajan

A newlatex protein named nivulian-I of plant origin has been isolated and purified from the latex of Euphorbia nivulia Buch.-Ham., a member of Euphorbiaceae family; by using centrifugation, acetone precipitation and ion exchange chromatography. This protein was characterized by sodium dodecyl sulphate polyacrylamide gel electrophoresis and mass spectroscopic analysis. Molecular mass of nivulian-I is 31486.985 Da. Peptide mass fingerprint revealed that, it fairlymatches with Tubulin alpha-1 chain (TBA1_ELEIN) of Eleusine indica (L.) Gaertn, a member of Poaceae family. This investigation infers that nivulian-I may be a novel protein of plant origin.