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Calculation of equilibriumintrinsic binding constant and cooperativity coefficient of polynucleotide poly(I)-poly(C) protonation

A.G.Kudrev

In the present work the pH-induced formation of double stranded poly(inosinic)–poly(cytidylic) acid has been studied by means of the hard modelling method based on the results of multivariate data analysis soft modelling method. The equilibrium model, describing species distribution changes as functions of solution acidity has been proposed. Proposed approach has been used to determine intrinsic protonation constants of poly(I-)–poly(C) polynucleotide system in aqueous solutions such as cooperativity coefficient (= 6.0) and a proton binding constant (lgKg = 8.92).