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Assessment and optimization of pesticide degradation by Pseudomonas putida

C.Arunachalam, A.M.Velmurugan

Number of bacteria species isolated fromthe highly pesticide pollution soil. Among the several isolate Pseudomonas putida grown well on the various pesticide containing medium (Pentachlorophenol and Chlorpyrifos) produced yellow color colonies, pesticide used as sole source of carbon energy and releasing chloride compound. Biodegradation of pesticide were optimized.Maximum degradation was found on the glucose substrate with co-substrate pantothenic acid and pH of 7 was found to be optimum and the P. putida degraded 70%and 75%, Pentachlorophenol and Chlorpyrifos respectively at 37ºC±2 for 10 days.