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Asimplified and small-scale sample preparation technique for determining malachite green and leuco-malachite green in cultured eel

Naoto Furusawa

Aninexpensive, simple, and small scale technique of sample preparation followed by high-performance liquid chromatography (HPLC) coupled photodiode array (PDA) detector for simultaneous quantification of malachite green (MG) and itsmetabolite, leuco-malachite green (LMG), in cultured eel is described. TheHPLC-PDAwas performed on aC4columnwith anisocratic mobile phase. Analytes were extracted from the sample using a handheld ultrasonic homogenizer, and purified by MonoSpin®C18, a centrifugal monolithic SPE spin mini-columns.The proposed method was validated by the analyses of spiked eel samples, resulting recoveries > 95%with relative standard deviations<2%and total analytical time <15min/sample (24 samples <4 hours).