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Antioxidant activity and chemical analysis of Mentha spicata cultivated from west northern region of Algeria by headspace solid phase micro-extraction and hydro-distillation

Hocine Allali, Ilyas Chikhi, Mohamed El Amine Dib, Alain Muselli, Nadia Fekih, Nawel Meliani, Mohammad A.Kamal, Boufelfja Tabti, Jean Costa

The essential oil of a Mentha spicata L. (collected fromAlgeria) was extracted by hydrodistillation and solid phasemicro-extraction (SPME). The oils have been studied by GC and GC-MS. Thirty seven compounds identified in the aerial parts oil extracted by hydrodistillation, the principal components being carvone (48.42%), eucalyptol (17.6%) and neoisodehydrocarveol acetate (11.7%). On the other hand, the oil extracted by SPME showed eucalyptol (55.1%) as the principal componentwithmoderate amounts of carvone (7.2%), (Z)-dehydro-carvone (4.3%), cis-carveol (3.9%) and carvacrol (3.0%). In addition, it should be noted that 11 compounds identified only in the volatile fractions extracted using HS-SPME and not indentified in essential oils. Isolated essential oil was tested for radical-scavenging ability using the stable DPPH radical assay, which showed concentration-dependant antiradical activities, i.e. a percent of inhibition of 52.21%in the presence of 12.6 mg/mL.