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Analysis of andrographolide in some ayurvedic formulations byHPTLC

N.Mahadevan, Somesh Thapliyal, Rakesh Garg, M.J.Nanjan

A HPTLCmethod to determine andrographolide inAndrographis paniculata and some ayurvedic herbal formulations was developed. The chromatographic separationwas performed on silica gel F254 precoatedHPTLCplates. Ethyl acetate-methanol-glacial acetic acid (8:1:0.2)was used asmobile phase. Rf value of andrographolidewas 0.64.Calibration plots of peaks area against concentrationwere linear in the range of 0.6 to 3.6 ìg. The limit of quantification was 0.60g and limit of detection was 0.20g. The amount of andrographolide in Andrographis paniculata powder, formulation I and formulation II was found to be 0.82%, 0.56%and 0.44%respectively. The developed HPTLCmethod is accurate, precise, simple, rapid and selective.