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A RP-HPLC Method for Estimation of Tramadol HCl and Chlorzoxazone in Tablet Formulation-Validation

Dutta M and Wakode S

A simple, rapid and selective method involving reverse phase liquid chromatography is developed for the concurrent determination of Tramadol HCl and Chlorzoxazone in tablet formulation. The chromatographic parameters involved using a Promosil C18 column with dimensions 4.6*250 mm, 5 μ and Acetonitrile and 0.1% Trifluroacetic acid in water in the ratio 60:40 (v/v) as the mobile phase. The flow rate set was of 1.0 mL/min and detected at 275 nm using UV detector. The linearity of Tramadol HCl and Chlorzoxazone were in the range of 25.00 μg/mL-45.00 μg/mL and 125.00 μg/mL-225.00 μg/mL respectively. Standard addition method was used to calculate percentage recovery. The average amount of recovery was found to be 98.8% and 99.7% for Tramadol HCl and Chlorzoxazone respectively. The method was found to be accurate and precise for determining both the drugs simultaneously.