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A Novel 1, 10-Phenanthroline-Based Fluorescent Probe for Selective Detection of D-3-HB

Chunlei Guo1, Mengyi Xu1, Cheng Chen1, Zhen Zheng1, Juan Chen1, Rongqing Geng1 and Qingming Wang1,2*

A new probe, 4-(1H-imidazo[4,5-f] [1,10] phenanthrolin-2-yl) nitrobenzene, has been synthesized and characterized by 1H-NMR, 13C-NMR, EAs, FTIR, ESI-MS. UV-Vis and fluorescence spectrum results shown that probe 1 was highly selective to D-3-HB in DMSO/H2O=1:1 (v:v=1:1), instead of common anions and metal ions. The absorbance intensity and the colour of probe 1 solution increased gradually with the increase of D-3-HB concentration and two new absorption bands appeared at 294 nm and 360 nm. The results showed that probe 1 can be a good candidate for simple, rapid and sensitive probe for the detection of D-3-HB.