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A new RP-HPLC-UV method for the simultaneous quantification of aceclofenac in bulk & its tablets

P.M.Chatrabhuji, Chintan V.Pandya,M.C.Patel

Asimple, selective, linear, precise, and accurate RP-HPLCmethod was developed and validated for the estimation of Aceclofenac from bulk drug. Chromatographic separation was achieved isocratically on a Phenomenex, C8 column (250×4.6 mm, 3 µ particle size) using a mobile phase, (0.01M ammoniumacetate bufferwith 2ml triethylamine, (v/v)-acetonitrile (68:32 v/ v) pH was adjusted to 6.5 with glacialAcetic acid. The flowrate was 1.2 ml/ min and effluent was detected at 270 nm and 20ìl of sample was injected. The retention time of Aceclofenac was 6.4 min. Linearity was observed in the concentration range of 8-16 µg/ml. Percent recoveries obtained for Aceclofenac was 99.65-99.93%. The percentage RSD for precision and accuracy of the method was found to be less than 1%. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision, LOD and LOQ. The method developed was successfully applied for the analysis& estimation ofAceclofenac in bulk drug.