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A Brief note on Glycoprotein Connecting Cells

Michel Richard

Glycoproteins are troublesome to crystallize since they have heterogeneous glycans composed of numerous monosaccharides with impressive rotational opportunity almost their O-glycosidic linkages. Crystallographers examining N-glycoproteins regularly balk this issue by utilizing β1, 2-N-acetylglucosaminyltransferase I (MGAT1)–deficient mammalian cell lines, which deliver recombinant glycoproteins with youthful N-glycans.